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Immunostaining After organotypic culture or cryosection, the cochlear explants or tissue sections were fixed with 4% PFA, permeabilized with 1% TritonX-100 in PBS, and blocked by incubation in PBT-1 solution (0.1% Triton X-100, 8% donkey serum, 1% bovine serum albumin, and 0.02% sodium azide in PBS) at room temperature for 1 h

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Jens Fisher and Alex Haas (Roche Pharma Research and Early Development) and cultured in DMEM supplemented with 10% HI-FCS, 25 g/mL streptomycin, 25 U/mL penicillin, and 300 g/mL G418 (Sigma-Aldrich)

Without specific training in functional or environmental medicine, most providers dont have the clinical framework to connect multi-system symptoms to a single environmental driver